NAD+ 500MG

Nicotinamide Adenine Dinucleotide (NAD⁺) is a universal redox cofactor and signaling substrate central to bioenergetics, genome maintenance, and protein deacylation. The 500 mg research format is designed for high-capacity experimental workflows interrogating redox flux, mitochondrial function, and NAD⁺– dependent enzyme biology across cell, tissue, and ex vivo models.

Redox Carrier: NAD⁺/NADH couples power dehydrogenase reactions in glycolysis, the TCA cycle, and oxidative phosphorylation, controlling electron transfer and ATP output.
Enzymatic Substrate: Consumed by Sirtuins (NAD-dependent deacylases), PARPs (DNA-damage signaling), and CD38/CD157 ectoenzymes (Ca²⁺ and immunometabolic signaling).
Compartmentalization: Nuclear, cytosolic, and mitochondrial NAD⁺ pools are maintained via the NAMPT salvage axis (NAM → NMN → NAD⁺), linking nutrient status to repair and longevity programs in research systems.

Mitochondrial respiration and bioenergetics • PARP activity and DNA-damage response • Sirtuin/epigenetic regulation • Redox biology and ROS handling • Immunometabolic CD38/CD157 pathways • Aging and stress-adaptation models • NAD⁺ salvage kinetics and precursor comparisons (NMN/NR).

Revitalized Health compounds are prepared under cGMP-aligned conditions using pharmaceutical-grade inputs. Each NAD⁺ lot is confirmed at ≥99% purity by HPLC with identity verified via LC-MS/MS. Each batch includes appearance and pH/osmolality checks (where applicable) and microbial/endotoxin screening to research-grade specifications. Batch-specific Certificates of Analysis provide full analytical parameters and results.

Store lyophilized NAD⁺ at 2–8 °C, protected from light and moisture. Reconstitute immediately prior to use with suitable diluent; maintain at 2–8 °C and utilize within 20 days. Avoid repeated freeze–thaw cycles and exposure to elevated temperature or alkaline pH to limit hydrolysis. For the 500 mg format, prepare working aliquots to minimize headspace and handling during multi-experiment series.

For laboratory research use only. Not intended for human consumption, therapeutic, or diagnostic application. Supplied exclusively to qualified professionals conducting controlled scientific investigations.

Identity: Nicotinamide adenine dinucleotide (oxidized form, NAD⁺), ubiquitous pyridine nucleotide coenzyme.
Mode: Central hydride carrier in glycolysis, TCA, and ETC (Complex I). Serves as an obligate substrate for sirtuins (SIRT1–7), PARPs, and CD38/CD157 ectoenzymes. Exogenous NAD⁺ and precursors augment cellular pools via salvage pathways (NAMPT/NMNAT1-3); nuclear/cytosolic/mitochondrial compartmentalization involves NMNAT isoforms and SLC25A51-mediated mitochondrial import. Functional consequences include shifts in NAD⁺/NADH redox, increased deacetylation signaling, enhanced mitochondrial biogenesis programs (PGC-1α axis), and DNA repair enzyme activity in models.
Analytics: Identity/purity by HPLC/LC-MS; targeted metabolomics of NAD metabolome (NAD⁺, NADH, NMN, NR, NAM) by LC-MS/MS; sirtuin activity assays, PARP activity/ADP-ribosylation; high-resolution respirometry (OXPHOS/ETC coupling), NADH fluorescence lifetime imaging (FLIM). Monitoring: tolerability (flushing, chest pressure with rapid infusions), GI upset, sleep disturbance reports; methylation demand context via homocysteine/folate/B12 when high precursor loads are used.